Development of a 20plex STR multiplex panel

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Karadayi H., Bulbul Ö., Filoglu G.

Forensic Science International: Genetics Supplement Series, cilt.6, 2017 (ESCI, Scopus)

• Yayın Türü: Makale / Tam Makale
• Cilt numarası: 6
• Basım Tarihi: 2017
• Doi Numarası: 10.1016/j.fsigss.2017.09.083
• Dergi Adı: Forensic Science International: Genetics Supplement Series
• Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, Criminal Justice Abstracts, EMBASE
• Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
• İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet

Özet

© 2017 Elsevier B.V.We developed a single reaction, fluorescently labeled in-house STR panel containing 19 autosomal loci plus Amelogenin for forensic use. We chose 18 loci from two commercial kits (AmpFlSTR® Identifiler®, Thermo Fisher Scientific, and The PowerPlex® 16 System, Promega) and combined with two mini-STRs (D6S1043 and D12S391) that do not exist in either of the kits to constitute a 20plex panel. The 20plex system is evaluated by specificity, sensitivity, precision and accuracy, analytical threshold, stochastic threshold, heterozygous balance, case-type samples, and PCR-based studies. The results demonstrate that 20plex STR multiplex panel is a robust, reliable and valuable assay for forensic applications.