Current Issues in Antioxidant Measurement

Apak R.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol.67, no.33, pp.9187-9202, 2019 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 67 Issue: 33
  • Publication Date: 2019
  • Doi Number: 10.1021/acs.jafc.9b03657
  • Journal Name: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.9187-9202
  • Keywords: antioxidant activity, total antioxidant capacity, reactive species, antioxidant measurement, physical chemistry of antioxidants, current issues, ABSORBENCY CAPACITY ASSAY, ABTS RADICAL-CATION, ACTIVITY CAA ASSAY, ELECTRON-TRANSFER, LIPID-PEROXIDATION, OXIDATIVE STRESS, REACTIVE OXYGEN, IN-VITRO, PROOXIDANT ACTIVITY, TOTAL PHENOLICS
  • Istanbul University-Cerrahpasa Affiliated: No

Abstract

The rationale and scope of the main issues of antioxidant measurement are presented, with basic definitions and terms in antioxidant research (such as reactive species and related antioxidative defenses, oxidative stress, and antioxidant activity and capacity) in a historical background. An overview of technical problems and expectations is given in terms of interpretation of results, precision and comparability of methods, capability of simulating physical reality, and analytical performance (sensitivity, selectivity, etc.). Current analytical methods for measuring antioxidant and antiradical activity are classified from various viewpoints. Reaction kinetics and thermodynamics of current analytical methods are discussed, describing physicochemical aspects of antioxidant action and measurement. Controversies and limitations of the widely used antioxidant assays are elaborated in detail. Emerging techniques in antioxidant testing (e.g., nanotechnology, sensors, electrochemistry, chemometry, and hyphenated methods) are broadly introduced. Finally, hints for the selection of suitable assays (i.e., preferable for a specific purpose) and future prospects are given.