Detection of IgA producing cells in sheep intestine using immunoperoxidase staining Koyunlarin Barsaǧinda, Immunoglobulin A Sentezleyen Plazma Hücrelerinin Immunoperoksidaz Yöntemi ile Belirlenmesi

Yilmaz H.

Turkish Journal of Veterinary and Animal Sciences, cilt.20, sa.2, ss.73-77, 1996 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 2
  • Basım Tarihi: 1996
  • Dergi Adı: Turkish Journal of Veterinary and Animal Sciences
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.73-77
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır

Özet

Samples from the duodenum, jejunum and ileum of 6 sheep were taken and fixed in formol saline. Sections from these tissues were used in immunocytochemistry studies. Plasma cells producing IgA in the small intestine were determined with rabbitanti ovine IgA using immunoperoxidase staining. Cells with brown staining in 10 randomly selected area under microscope were counted using image analyser. Mean cell count of 10 microscope area of duodenum, jejunum and ileum was 43, 53.3 and 74 respectively.