Akademik Veri Yönetim Sistemi
8th International Congress of Molecular Medicine , İstanbul, Türkiye, 9 - 12 Kasım 2021, ss.15, (Özet Bildiri)
Objective: It is recommended that vascular risk factors
play an curicial role in the pathophysiology of Alzheimer's
Disease (AD) and platelets are efficient in this process. In
this study, we planned to investigate the plasma levels of
Glycoprotein 1b(GP1b) proteins and von Willebrad
Factor (VWF) in AD patients and healthy individuals to
determine platelet aggregation and the relationship of
platelets with endothelium. In order to investigate the
regulation of GP-1b activation which is one of the
proteins that associate between the platelet and
endothelium, at the molecular level, we detected
mir26a-5p, which we think may be effective in this
pathway, and the von Willebrand Factor at the molecular
level, by detecting mir24-3p levels with qRT-PCR, and
determining their relationship with the disease, intended
to be determined.
Materials-Methods: 23 patients and 35 controls were
included in the study. Plasma GP1b and vWF levels were
determined by ELISA. Platelet functions were studied
with a lumiagregometer device by giving ADP stimulus.
miR26a-5p and miR24-3p levels were determined by
qRT-PCR.
Results: In our study, platelet aggregation % amplitude
value (p=0.028) and GP1b levels (p=0.107) were found to
be higher in the patient group than controls. MMSE(p=0.001) and hsa-mir26-5p (p=0.043) were found
to be lower in the patient group than controls.
Conclusion: High platelet aggregation % amplitude value
in the patient group indicates an increase in platelet
functions. Low levels of hsa-mir26-5p expression may be
associated with increased GP1b levels in the Alzheimer’s
Disease.
Keywords: Alzheimer's Disease, ADP, Platelet
Aggregation, GP1b, miR26-5p