A new approach in the identification of degraded paternity samples

ÖZCAN, Sevil; Petridis, Gabriel; Yukseloglu, EMEL; Kocias, Yani; Kalfoglu, Ersi; Atasoy, Sevil

doi:10.1016/j.fsigss.2009.09.030

A new approach in the identification of degraded paternity samples

ÖZCAN S., Petridis G., Yukseloglu E. H., Kocias Y., Kalfoglu E. A., Atasoy S.

Forensic Science International: Genetics Supplement Series, cilt.2, sa.1, ss.174-175, 2009 (ESCI, Scopus)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 2 Sayı: 1
Basım Tarihi: 2009
Doi Numarası: 10.1016/j.fsigss.2009.09.030
Dergi Adı: Forensic Science International: Genetics Supplement Series
Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, Criminal Justice Abstracts, EMBASE
Sayfa Sayıları: ss.174-175
İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet

Özet

DNA extraction from bone is an important issue particularly in paternity cases when bones are the only remaining material to obtain and analyze DNA. The difficulties arising from bacterial damages, taphonomic factors and diagenesis might negatively affect the extraction and the amplification of DNA. This makes the laboratory procedure a hard and time-consuming process, and the analysis can fail. Analyzing mini-STRs in this type of degraded samples is highly recommended. In this study a new extraction technique was carried on bone samples which were then typed formini-STRs. The aim was to differentiate two genetically related skeletons found in the same familial grave for a paternity test. The analysis revealed that this new extraction technique along with mini-STR analysis can properly be an effective way to obtain and analyze DNA in bones in the field of forensic sciences. (C) 2009 Elsevier Ireland Ltd. All rights reserved.