Akademik Veri Yönetim Sistemi
2nd. International Food Chemistry Congress, Antalya, Türkiye, 16 - 19 Mart 2023, cilt.3, ss.54-55, (Özet Bildiri)
Drug-facilitated crimes (DFC) are defined as crimes such as robbery, sexual assault, money extortion or battery when committed while the victim is under the influence of drugs1 . Diazepam (DIA) and Flunitrazepam (FLU) are frequently used in drug-facilitated crimes, due to their short duration of action and easy availability2 . Tasteless, colorless and soluble character of these substances, makes it easier to commit crimes by added to the drink or food of the victims3 . It is crucial to develop new methods that can evaluate the presence of drugs frequently use in DFC crimes in alcohol residues or contaminated material to prove acts connected with crime4 . This work aims to develop a technique using high-performance thin-layer chromatography (HPTLC) for the simultaneous determination of DIA and FLU in spiked beverages. Although DIA can be detected in various beverages by HPTLC in previous studies2 , this developed method can determine DIA and FLU together in spiked beverage samples simultaneously for the first time. Moreover, HPTLC has an advantage which allows samples to be analyzed directly, while current chromatographic methods require sample pretreatment to detect these two substances. The HPTLC setup used included an automatic TLC Sampler 4, an automatic developing chamber, a TLC Scanner 3, and precoated silica gel 60 F254 HPTLC glass plates (20x10cm). The bandwidth was each adjusted at 6 mm and the injection volume was 2 µL. The loaded plates were developed up to 80 mm with 7 mL Hexane-Ethyl Acetate-Glacial Acetic Acid (7:2,5:0,5) mobile phase in an unsaturated twin trough glass chamber; they were dried for 5 min. Bands were quantified in single-wavelength reflectance mode and evaluation was carried out by measuring the peak areas with linear regression. Multi-wavelength scanning was performed in the 200–400 nm range at 20 nm s–1 with a data resolution of 1 nm per step. The linearity range was between 6-50 ng/spot for both drug substances. By using this method, it will be possible to identify and analyze the evidence found at the crime scene which contaminated with these two active substances in trace amounts. This study is capable of providing valuable information for routine analysis, and it will serve the interests of justice by verifying the findings.