Fabrication and sensing properties of phenolphthalein based colorimetric and turn-on fluorogenic probe for CO32- detection and its living-cell imaging application

Elmas Ş. N., Karagoz A., Aydin D., Arslan F. N., Sadi G., Yilmaz I.

TALANTA, cilt.226, 2021 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 226
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.talanta.2021.122166
  • Dergi Adı: TALANTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, L'Année philologique, Aerospace Database, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, Linguistic Bibliography, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Phenolphthalein, Fluorescent sensor, Carbonate, Density functional theory, Living cell, SCHIFF-BASE, FLUORESCENT SENSOR, CHEMICAL SENSOR, ANION RECEPTORS, CARBON-DIOXIDE, OPTICAL SENSOR, SOIL SAMPLES, ION, ACETATE, LIGAND
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır

Özet

Herein, an easy assembled colorimetric and ''turn-on'' fluorescent sensor (probe P4SC) based on phenolphthalein was developed for carbonate ion (CO32-) sensing in a mixture of EtOH/H2O (v/v, 80/20, pH = 7, Britton-Robinson buffer) media. The probe P4SC demonstrated high sensitive and selective monitoring toward CO32- over other competitive anions. Interaction of CO32- with the probe P4SC resulted in a significant increment in emission intensity at lambda(em) = 498 nm (lambda(ex) = 384 nm) due to the strategy of blocking the photo induced electron transfer (PET) mechanism. H-1 NMR titration and Job's methods, as well as the theoretical study were carried out to support the probable stoichiometry of the reaction (1:2) between P4SC and CO32-. The binding constant of the probe P4SC with CO32- was calculated as 2.56 x 10(10) M-2. The probe P4SC providing rapid response time (similar to 0.5 min) with a satisfactorily low detection limit (14.7 nM) may be useful as a valuable realistic sensor. The imaging studies on the liver cancer cells (HepG2) shows the great potential of the probe P4SC for the sensation of intracellular CO32- anions. Furthermore, the satisfactory recovery and RSD values obtained for water application confirming that the probe P4SC could be applied to sensing of CO32- ion.