Akademik Veri Yönetim Sistemi
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.74, sa.1, ss.71-78, 2017 (Scopus, TRDizin)
We report a case of distal subungual onychomycosis of the thumb of the right foot of a 25-year-old female. Nail examination was examined in our laboratory anddistal subungual onycomycosis was detected. In direct microbiological examination septate hyphae was observed by using %20 KOH. Scraping of nail was cultured on two Sabouraud's Dextrose Agar with cycloheximide and without cycloheximide slants at 25 °C for one week. Growth of colony was observed in without cycloheximide slants. The same brown colonies were produced on repeated cultures by using Sabouraud's Dextrose Agar without cycloheximide. After growth of colony, slide cultures were prepared and brown-colored septated hyphae, perithecia, lemon- shaped ascospores were observed by light microscopy. The causative agent was identified as Chaetomium globosum based on DNA sequencing andmycological examination. Using M38-A2 microdilution method, minimum inhibitory concentration values of, amphotericin B fluconazole, itracanozole, miconazole, ketoconazole, flucytosine voriconazole were determined as 4- > 64, 1-0.125, 0.125- > 64-0.5 μg/mL, respectively. Fluorocytosine and fluconazole were determined as resistant for Chaetomium globosum and the best effective antifungal was determined as miconazole and ketoconazole. The patient was treated by using oral itraconazole (250 mg /a day) and local application of amorolfine 5% nail lacquer for 12 weeks.