Identification and Anti-oxidant Capacity Determination of Phenolics and their Glycosides in Elderflower by On-line HPLC-CUPRAC Method

Celik S. E. , Ozyurek M. , Gueclue K., Capanoglu E., Apak R.

PHYTOCHEMICAL ANALYSIS, vol.25, no.2, pp.147-154, 2014 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 2
  • Publication Date: 2014
  • Doi Number: 10.1002/pca.2481
  • Page Numbers: pp.147-154
  • Keywords: On-line HPLC-CUPRAC assay, post-column derivatisation, total anti-oxidant capacity, elderflower, SAMBUCUS-NIGRA, LIQUID-CHROMATOGRAPHY, SCREENING METHOD, L., FLAVONOIDS, EXTRACTS, ACIDS, CHEMILUMINESCENCE, POLYPHENOLS, OXYGEN


IntroductionDevelopment and application of an on-line cupric reducing anti-oxidant capacity (CUPRAC) assay coupled with HPLC for separation and on-line determination of phenolic anti-oxidants in elderflower (Sambucus nigra L.) extracts for their anti-oxidant capacity are significant for evaluating health-beneficial effects. Moreover, this work aimed to assay certain flavonoid glycosides of elderflower that could not be identified/quantified by other similar on-line HPLC methods (i.e. 2,2-diphenyl-1-picrylhdrazyl and 2, 2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid).