Akademik Veri Yönetim Sistemi
MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE, cilt.62, sa.3, ss.281-284, 2006 (SCI-Expanded, Scopus)
The study investigated the effects of three different KH2PO4 concentrations (0.59 mM; 1.19 mM; 2.38 mM) in Whitten's medium and co-culture on in vitro two-cell blocks and their development to the blastocyst stage of inbred BALB/C mice embryos following in vitro fertilization.
Standard IVF and IVC procedures were used and mouse oviduct cells were used as a co-culture. The results demonstrated that various concentrations of potassium were not effective on the cleavage rates. 1.19 mM KH2PO4 with and without co-culture groups (P < 0.05)had the highest rate of reaching the 4-to 6- and 8-cells. At the 72(nd) hour, the 1.19 mM KH2PO4 groups without (55.26%) and with co-culture (44.77%) (P < 0.05) displayed the most satisfactory development to the morula stage. Although 0.59 mM and 2.38 mM KH2PO4 were found to be insufficient to develop up to morula, 0.59 mM KH2 PO4 with co-culture may have beneficial effects (31.57%; P < 0.05). On the other hand, despite the high concentration of KH2PO4 (16.90%) supporting a more effective development rate to the morula than the lower level (P < 0.05), it was noted that co-cultures having a high KH2PO4 were not beneficial. KH2PO4 with and without (30.00%) co-culture (26.01%) (P < 0.05) demonstrated the most beneficial development of the blastocyst stage.