Antioxidant Activity/Capacity Measurement. 2. Hydrogen Atom Transfer (HAT)-Based, Mixed-Mode (Electron Transfer (ET)/HAT), and Lipid Peroxidation Assays

Apak R. , Ozyurek M. , Guclu K., Capanoglu E.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol.64, no.5, pp.1028-1045, 2016 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Review
  • Volume: 64 Issue: 5
  • Publication Date: 2016
  • Doi Number: 10.1021/acs.jafc.5b04743
  • Page Numbers: pp.1028-1045
  • Keywords: hydrogen atom transfer assays, mixed-mode assays, lipid peroxidation assays, antioxidant mechanisms, food analytical methods, RADICAL ABSORBENCY CAPACITY, FLOW-INJECTION ANALYSIS, FLUORESCENCE-BASED ASSAY, CROCIN BLEACHING ASSAY, SCAVENGING CAPACITY, COUPLED ELECTRON, RATE CONSTANTS, DPPH ASSAY, VITAMIN-E, IN-VITRO


Measuring the antioxidant activity/capacity levels of food extracts and biological fluids is useful for determining the nutritional value of foodstuffs and for the diagnosis, treatment, and follow-up of numerous oxidative stress-related diseases. Biologically, antioxidants play their health-beneficial roles via transferring a hydrogen (H) atom or an electron (e(-)) to reactive species, thereby deactivating them. Antioxidant activity assays imitate this action; that is, antioxidants are measured by their H atom transfer (HAT) ore transfer (ET) to probe molecules. Antioxidant activity/capacity can be monitored by a wide variety of assays with different mechanisms, including HAT, ET, and mixed-mode (ET/HAT) assays, generally without distinct boundaries between them. Understanding the principal mechanisms, advantages, and disadvantages of the measurement assays is important for proper selection of method for valid evaluation of antioxidant properties in desired applications. This work provides a general and up-to-date overview of HAT-based, mixed-mode (ET/HAT), and lipid peroxidation assays available for measuring antioxidant activity/capacity and the chemistry behind them, including a critical evaluation of their advantages and drawbacks.