Akademik Veri Yönetim Sistemi
FOODS, cilt.13, sa.7, 2024 (SCI-Expanded, Scopus)
Antimicrobial resistance is one of the major public health problems worldwide. This study aimed to detect the presence of extended-spectrum beta-lactamase-(ESBL-)producing Escherichia (E.) coli in chicken meat in Istanbul, T & uuml;rkiye. Raw chicken meat samples (n = 208) were collected from different sale points and analyzed for ESBL-producing E. coli. In total, 101 (48.5%) isolates were confirmed as E. coli by PCR, of which 80/101 (79.2%) demonstrated multiple antibiotic resistance. Resistance against amoxicillin-clavulanic acid was most frequent (87.1%). Eighteen isolates (17.8%) demonstrated phenotypical ESBL resistance, as assessed by the double disc synergy test (DDST). Isolates were tested for the presence of beta-lactamase genes and mobilized colistin-resistant genes. The bla(TEM) group was most frequently detected (97.02%), followed by bla(CTX m) (45.5%), bla(SHV) (9.9%), and bla(OXA-2) (0.9%). However, mcr genes and bla(NDM,) bla(KPC), bla(VIM), and bla(OXA-48) genes were not found in any isolate. E. coli strains were tested for biofilm formation in six different media [Nutrient broth, LB broth, Tryptone Soya broth (TSB), TSB containing 1% sucrose, TSB containing 0.6% yeast extract, and BHI]. Biofilm formation by E. coli isolates (44/101, 43.5%) was highest in TSB with 1% sucrose. It is worth noting that all biofilm-producing isolates were found to harbor the bla(TEM-1) gene, which can indicate a high level of antibiotic resistance. This is the first report about ESBL-producing E. coli in poultry meat, the exposure of consumers in Istanbul metropolitan areas, and the ability of E. coli from this region to produce biofilms.