Isolation and preparation of monoclonal antibody to ovine Immunoglobulin M
TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.23, sa.2, ss.135-140, 1999 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 23 Sayı: 2
- Basım Tarihi: 1999
- Dergi Adı: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.135-140
- İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır
Özet
Ovine immunoglobulin M was isolated from sheep serum using gel filtration and affinity chromatography. Polyclonal and monoclonal antibodies against this isotype were prepared. The specificity of the monoclonal antibody was investigated using SDS-Page, immunoblotting, an enzyme-linked immunosorbent assay (ELISA) and affinity chromatography. On immunoblotting, monoclonal anti-IgM reacted with a 70 kD protein which corresponded to ovine IgM.