Akademik Veri Yönetim Sistemi
56th European Renal Associaton-European Dialysis Transplantation Association (ERA-EDTA) Congress, Budapest, Macaristan, 13 - 16 Haziran 2019, cilt.34, ss.214, (Tam Metin Bildiri)
URINARY EXOSOMAL MIRNA LEVELS REVEAL NEW
POTENTIAL BIOMARKERS IN TYPE 2 DIABETIC
NEPHROPATHY AND FSGS
Sinan Trabulus1, Mehmet Seyit Zor2, Eda Tahir Turanli2, Tamer Dincer1,
Selma Alagoz3, Meral Mese4, Erkan Yilmaz1, Nurhan Seyahi1
1Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Istanbul, Turkey, 2Istanbul
Technical University, Faculty of Science and Letters, Istanbul, Turkey, 3Bagcilar Training
and Research Hospital, Istanbul, Turkey and 4Kartal Lutfi Kirdar Training and Research
Hospital, Istanbul, Turkey
INTRODUCTION: We aimed to explore the diagnostic values of mir-192 and mir-145
miRNAs in patients with diabetic nephropathy (DN) and Focal Segmental
Glomerulosclerosis (FSGS).
METHODS: Patients aged between 40-65 years who had 24-h protein excretion > 1.0
g/day and creatinine clearance > 30 ml/min were included in this study. Urine samples
are collected from 9 patients (1 F, 8M; mean age 54.661.5) with Type 2 diabetes
mellitus, 7 patients (6 F, 1M; mean age 55,1+7,8) with FSGS and 4 healthy individuals
(1 F, 3M; mean age 48.264.8). Isolation of target miRNAs mir-192 and mir-145 were
performed using targeted assays from urinary exosomes then quantitative profiling of
isolated miRNAs performed by quantitative
RESULTS: Creatinine clearance and 24-h protein excretion were found as 45.6624.3
ml/min and 6.6763.17 g/day in Type 2 diabetic patients and as 72,5631,9 ml/min and
2,4761,33 g/day in FSGS patients, respectively. Real Time-PCR data were obtained
from patients with healthy controls. All target CT values were normalized according to
housekeeping gene and 2^-DCT values were calculated. Comparative 2^-DCT values
between data groups are shown in Figure 1. mir-145 levels were found to be
upregulated in DN (mean 2^-DCT 0.5660,2) compared to FSGS (mean 2^-DCT
0.0960,03) and healthy group (mean 2^-DCT 0.0860,06). Also mir-192 levels were
found to be upregulated in DN (mean 2^-DCT 3.1162) compared to FSGS (mean 2^-
DCT 1.3261) and healthy group (mean 2^-DCT 2.6561.2). mir-145 levels were found
to be upregulated in DN (mean 2^-DCT 0.5660,2) compared to FSGS (mean 2^-DCT
0.0960,03) and healthy group (mean 2^-DCT 0.0860,06). Also mir-192 levels were
found to be upregulated in DN (mean 2^-DCT 3.1162) compared to FSGS (mean 2^-
DCT 1.3261) and healthy group (mean 2^-DCT 2.6561.2). However, there were no
statistically significant differences among groups.
CONCLUSIONS: We have seen a trend in increase of both mir-145 and mir-192 levels
in DN patients compared to healthy group. We are further analyzing our data with
increased sample size.