Akademik Veri Yönetim Sistemi
6th International Congress on Advances in Bioscience and Biotechnology, Aksaray, Türkiye, 25 - 29 Temmuz 2022, ss.54-60, (Tam Metin Bildiri)
Mammary gland tumors have the highest incidence among dogs. Lipid-rich carcinoma is very rare
histological types of mammary tumor. However, it is generally observed in young female dogs and the
success of current treatment option is limited. Therefore, innovative approaches are required for the
treatment of canine mammary tumors. Traf2 and Nck-Interacting (TNIK) is a member of the mitogenactivated serine/threonine protein kinase family regulates Wnt signaling pathway. Additionally, the
phosphatidylinositol-3-kinase (PI3K)/Akt and mammalian targeting of rapamycin (mTOR) signaling
pathways is crucial for many aspects of cancer cell growth. Therefore, we aimed to determine the effect of
NCB-0684 as a TNIK inhibitor on PI3K/Akt/mTOR signaling in canine lipid-rich carcinoma cells via
determination of Akt and mTOR mRNA levels with RT-PCR analysis. Our findings demonstrated that NCB0684 inhibited Akt and mTOR gene expression levels at higher concentration. However, the effectiveness of
TNIK inhibitor is changed dependently its concentration. Thus, our results claim that NCB-0684 could
suppress PI3K/Akt/mTOR signaling. However, further investigations need to clarify the inhibitory activity
of TNIK inhibitor on PI3K/Akt/mTOR signaling in canine mammary tumors.