Akademik Veri Yönetim Sistemi
Materials Today Chemistry, cilt.44, 2025 (SCI-Expanded, Scopus)
The objective of this study is to develop a novel L-arginine (L-Arg) based peptide dendrimers loaded with β-ionone (BI) as an anticancer agent and investigate their antiproliferative activity on cancer cells in vitro. L-Arg containing peptide dendrimers (G1-3) as the branching units and ethylene diamine (EDA) as the core molecule were synthesized and characterized by using FTIR, 1H NMR and GPC-SEC techniques for the first time. Their cytotoxicities were also evaluated using MTT assay in vitro. BI loaded G2 and G3 dendrimers based nanoformulations (nG2:BI, nG3:BI) were obtained for the first time, and their particle size (nm), PDI and zeta potential (mV) were determined. To compare the effect of folic acid (FA) as a common receptor, nanoformulation nFA-G3:BI obtained by coupling the FA with the selected dendrimer G3 was also prepared and characterized. In vitro cytotoxic effects of these nanoformulations on the POF cells as healthy cell and HeLa cell lines as model cancer cells were comparatively evaluated by MTT assay. Cell viability (%) and the IC50 value of these nanoformulations were determined. The results demonstrate that the nG2:BI, nG3:BI and nFAG3:BI have an effective antiproliferative activity on HeLa cells. G3 can be used as a BI carrier to HeLa cells without the FA. These findings show that L-Arg based peptide dendrimers synthesized in this study effectively carry the BI molecule to the HeLa cell. It was concluded that nG2:BI, nG3:BI and nFAG3:BI as the nanocarriers can provide an advantage in delivering the BI efficiently into cancer cells in nanobiotechnology.