Akademik Veri Yönetim Sistemi
INSAC Natural and Health Sciences (INHS-2020), İstanbul, Türkiye, 22 - 23 Mayıs 2020, ss.102-103, (Tam Metin Bildiri)
Studies have revealed that there are point mutations, duplications and deletions in mtDNA in different tissues of individuals. Among the deletions observed, the mt4977 mutation, which is located in nucleotide positions 8470-8482 and 13447-13459 and leads to the loss of 4977 base pairs, is the most common. This deletion leads to the disappearance of 8 genes encoding the subunits of respiratory chain complexes, and expected to inhibit oxidative function and decrease the level of ATP production. Mitochondrial ATP production is known to play an important role in platelet function. However, there is no information in the literature related to this. Since platelet activation plays an important role in the pathophysiology of disease in ischemic heart disease (IHD), we wanted to address the relationship between platelet function and mt4977 deletion in IHD. Platelet functions were assessed by ADP stimulation for the evaluation of secretion and aggregation. There was no statistically significant difference between the IHD and control groups in terms of slope (Ώ) and % amplitude values. Platelet ATP levels were determined by chemiluminimetric method. There was no significant difference (P>0.05). Mt4977 bp deletion studied with Realtime PCR and mitochondrial deletion was observed at different ratios in both groups. The relationship between mitochondrial deletion rates of patient and control group and ATP amounts in platelets was examined. When compared with % mitochondrial deletion rate of the IHD patient group and the amount of ATP in the platelets, there was a significant correlation in reverse direction (r: -0.70), which is a significant difference (P<0.005).