An Efficient Green Method for Recovery oF Polyphenols from Olive Tree (Olea Europaea) Leaves by means of Lactic acid-based Deep Eutectic Solvent

Creative Commons License

Toprakçı Yüksel İ., Şahin Sevgili S.

The 9th International Mediterranean Symposium on Medicinal and Aromatic Plants (MESMAP-9), Ankara, Türkiye, 3 - 05 Mayıs 2023, ss.189-200, (Tam Metin Bildiri)

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.189-200
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet

Özet

Abstract

Biowastes generated during the processing of industrial crops and products are often rich in bioactive compounds like polyphenols. Using biowastes to obtain natural antioxidants is a sustainable and environmentally friendly approach that can promote human health while reducing waste. Olive leaf, which is one of the by-products of the olive oil industry, was used as a raw material in this study due to its rich bioactive content. The current study is aimed at establishing an efficient green media, using an eco-friendly lactic acid-based deep eutectic solvent (DES) system, consisting of a hydrogen bond donor-HBD (glycerol) and hydrogen bond acceptor-HBA (lactic acic). The molar ratio of lactic acid-glycerol mixture was selected 1:1 depending on our previous study [1]. Ultrasound-assisted extraction (UAE) method, a green extraction method, has been used to recover natural antioxidants from olive leaves. The extraction time and amplitude were determined for ultrasound-assisted extraction of bioactiverich extract from olive leaves prior to routine studies. The Box–Behnken design type of the response surface method (RSM) was employed to investigate the effects of independent parameters (particle size, solid mass and water content in DES) on the total phenolic content (TPC). Bioactive properties of the olive leaf extracts were also assessed depending on total flavanoid content (TFC) and antioxidant activity measured by 2 different assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) methods.

Key Words: Natural antioxidants, biowaste, deep eutectic solvent, green extraction, Box-BehnkenRSM.

Reference

[1] Yücel, M., Şahin, S. An Eco-Friendly and Sustainable System for Monitoring the Oleuropein-Rich Extract from Olive Tree (Olea Europaea) Leaves. Biomass Convers. Biorefinery, 2022, 12 (1), 47– 60. https://doi.org/10.1007/S13399-021-01556-0/TABLES/12.