Akademik Veri Yönetim Sistemi
Etlik Veteriner Mikrobiyoloji Dergisi, cilt.31, sa.1, ss.75-81, 2020 (TRDizin)
Contagious ecthyma (CE) is a zoonotic viral infection and common localized cutaneous infection of young sheep and goats caused by a Parapoxvirus with worldwide distribution. The aim of this study was to determine whether rabbits will be used instead of lambs and kids in immune and safety studies after the production of CE vaccine. The titres of Pendik CE pathogen strain isolated from lamb (E(P)CK4) and attenuated vaccine strain originated from lamb (E(P)CK22) used in the study were TCID50 106.50 and 107.00/ml, respectively. In the study, to determine the pathogenicity of CE virus (E(P)CK4) in rabbits, it was found that CE virus-specific hyperemia, vesicles and pustules were not observed and there was no increase in body temperature, and CE virus was not detected by PCR in the scabs located in the skin of back-waist regions. In the blood sera of rabbits treated with pathogen CE virus and vaccinated with CE vaccine virus, no antibodies were detected against CE virus. In the immunity study in lambs and kids vaccinated with CE vaccine (E(P)CK22), it was found that the vesicles, pustules and scabs appeared on day 3 and the lesions healed on the 15th day seen that CE vaccine virus protects lambs and kids against pathogen CE (E(P)CK4) strain.