The Role of Quercetin in Alleviating the Testicular Oxidative Stress and Apoptosis in Streptozotocin-Induced Diabetes

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Hajiyeva R., Durmuş S., Tunçdemir M., Gelişgen R., Sözer V., Büyükçolpan Mirzataş E., ...Daha Fazla

Cerrahpaşa Medical Journal, cilt.45, sa.3, ss.159-166, 2021 (TRDizin)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 3
  • Basım Tarihi: 2021
  • Dergi Adı: Cerrahpaşa Medical Journal
  • Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.159-166
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet

Özet

Objective: The aim of this study was to investigate the effects of quercetin on oxidative stress and apoptosis in testicular tissue of streptozotocin-induced diabetic male rats.

Methods: Thirty-two Wistar type Albino rats were divided into 4 groups. The groups are planned as follows: group 1: control group

(only 0.9% sodium chloride, i.p. injection for 30 days, n = 8), group 2: DM group (streptozotocin: 50 mg/kg, freshly dissolved in

0.9% sodium chloride, i.p, n = 8), group 3: DM + QR group (quercetin: 20 mg/kg/day, diluted in 4% ethanol, 30 days, i.p.) (streptozotocin: 50 mg/kg, i.p., single dose, n = 8), group 4: control + QR group (quercetin: 20 mg/kg/day, 30 day, i.p., n = 8). Testicular tissue

samples were taken under anesthesia on the 31st day of the study. Malondialdehyde, lipid hydroperoxide, advanced oxidation protein

products, total thiol, nonprotein sulfhydryl compounds, and Cu,Zn-superoxide dismutase were determined by spectrophotometric

method.

Results: Malondialdehyde and lipid hydroperoxide levels were higher in the DM group compared to the control and DM+ QR

group. Advanced oxidation protein product levels were lower in the control group than in the control+ QR and DM group, while

total thiol, nonprotein sulfhydryl levels, and Cu,Zn-superoxide dismutase activity were higher in DM+ QR group compared to control

and DM groups. In the DM+ QR group, a significant decrease was found in the number of apoptotic cells compared to the DM group

(P < .001).

Conclusion: Quercetin administration can be potentially beneficial and effective in preventing oxidative stress (lipid and protein) damage

and apoptosis in the testicles of diabetic rats.