Akademik Veri Yönetim Sistemi
Cerrahpaşa Medical Journal, cilt.45, sa.3, ss.159-166, 2021 (TRDizin)
Objective: The aim of this study was to investigate the effects of quercetin on oxidative stress and apoptosis in testicular tissue of streptozotocin-induced diabetic male rats.
Methods: Thirty-two Wistar type Albino rats were divided into 4 groups. The groups are planned as follows: group 1: control group
(only 0.9% sodium chloride, i.p. injection for 30 days, n = 8), group 2: DM group (streptozotocin: 50 mg/kg, freshly dissolved in
0.9% sodium chloride, i.p, n = 8), group 3: DM + QR group (quercetin: 20 mg/kg/day, diluted in 4% ethanol, 30 days, i.p.) (streptozotocin: 50 mg/kg, i.p., single dose, n = 8), group 4: control + QR group (quercetin: 20 mg/kg/day, 30 day, i.p., n = 8). Testicular tissue
samples were taken under anesthesia on the 31st day of the study. Malondialdehyde, lipid hydroperoxide, advanced oxidation protein
products, total thiol, nonprotein sulfhydryl compounds, and Cu,Zn-superoxide dismutase were determined by spectrophotometric
method.
Results: Malondialdehyde and lipid hydroperoxide levels were higher in the DM group compared to the control and DM+ QR
group. Advanced oxidation protein product levels were lower in the control group than in the control+ QR and DM group, while
total thiol, nonprotein sulfhydryl levels, and Cu,Zn-superoxide dismutase activity were higher in DM+ QR group compared to control
and DM groups. In the DM+ QR group, a significant decrease was found in the number of apoptotic cells compared to the DM group
(P < .001).
Conclusion: Quercetin administration can be potentially beneficial and effective in preventing oxidative stress (lipid and protein) damage
and apoptosis in the testicles of diabetic rats.