Research Information System
2nd International Congress on Medical Sciences and Biotechnology, Uşak, Turkey, 1 - 03 October 2020, pp.379, (Summary Text)
Diabetes is a disease that caused the most death in 2016 according to World Health
Organization. It is predicted that 642 million people may have diabetes in 2040. For this reason,
diabetes remains a wide area of research interest in medical sciences. Myrtus communis L.
subsp. communis (MC) can grow on the entire coastline of the Mediterranean Aegean regions.
It is known as a medicinal herb for treatment of various diseases, such as inflammation, ulcers,
diarrhea, respiratory and digestive system disorders. The present study aims to investigate the
effects of MC leaf ethanol extract on small intestine damage of postmenopausal diabetic rats.
Female Sprague-Dawley rats were divided into 5 groups. Sham-operated control group: The
rats underwent surgical incision under anesthesia, ovaries were identified but not removed.
Ovariectomy group: Bilateral ovariectomy was performed and saline solution was given for 4
weeks. Diabetic group: Rats were treated with 45mg/kg i.p streptozotocin (STZ) (prepared in
pH 4.5 citrate buffer ) and given saline for 4 weeks. Ovariectomy + Diabetic group: Bilateral
ovariectomy was performed, and after one week of recovery rats were treated with STZ
(45mg/kg i.p) and given saline for 4 weeks. Ovariectomy + Diabetic + MC group: Bilateral
ovariectomy was performed and after one week of recovery, STZ (45mg/kg i.p) was given to
induce diabetes. 48 hours after STZ injection, MC extract (100 mg/kg) was given by oral gavage
for 4 weeks. At the end of the experiment, small intestine tissues were taken. Total antioxidant
(TAS), total oxidant (TOS), reactive oxygen (ROS), nitric oxide (NO), sialic acid (SA),
deoxyribonucleic acid (DNA) and advance oxidative protein products (AOPP) levels were
determined in small intestine tissue. In diabetic, ovariectomy and ovariectomy + diabetic
groups, ROS, TOS, NO, SA, DNA and AOPP levels were found to increase while TAS level
decreased. Administration of MC extract reversed these changes. These results indicated that
MC leaf extract had a protective effect on small intestine damage of postmenopausal diabetic
rats.