Akademik Veri Yönetim Sistemi
Journal of Molecular Structure, cilt.1358, 2026 (SCI-Expanded, Scopus)
The most critical problems today include the late diagnosis and late start of treatment of colorectal cancer and the varying response to chemotherapeutic agents in different patients. Therefore, the clinical implementation of methods to support early diagnosis and the development of various anti-cancer drugs is crucial. Palladium complexes offer potential advantages over cisplatin, including reduced systemic toxicity, increased selectivity towards cancer cells, and the potential to overcome mechanisms of cisplatin resistance. Therefore, this study aimed to investigate the effects of two previously developed palladium complexes (Pd1A and Pd1B) on the viability of colorectal cancer cells (HCT116) compared to cisplatin (CISP), and to examine the changes in biomolecules using advanced Fourier Transform Infrared Spectroscopy (FTIR) analyses. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay was used to determine cell viability, and Orange Data Mining software was used for advanced spectral analyses; Principal Component Analysis (PCA) was performed in RStudio (version 4.3.2) using the prcomp function. When the results were evaluated, Pd1A and Pd1B, which were more cytotoxic than CISP, showed alterations in cellular components, including structural and chemical changes, that affected nucleic acid integrity, protein conformation, and lipid organization. The results suggest that palladium complexes induce additional biomolecular changes in colorectal cancer cells compared to cisplatin, and that FTIR spectroscopy is an effective tool for monitoring these changes.