Escherichia coli O157 is one of the major threats to public health due to the consumption of meat and meat products. The microorganism is known as a food-borne pathogen even in the presence of low levels. Therefore, a more sensitive method is required to be used for the detection of the microorganism. Immunomagnetic separation (IMS) is a technique used to improve the sensitivity of the detection. IMS has been used to determine the presence of E. coli O157 in raw and cooked Doner samples in this study. 3 out of 30 raw Doner samples have been found to be contaminated with E. coli O157. None of the cooked samples were carrying the agent.