Akademik Veri Yönetim Sistemi
Etlik Veteriner Mikrobiyoloji Dergisi, cilt.31, sa.1, ss.7-19, 2020 (TRDizin)
Abstract: Enrofloxacin, linko-spectin and florfenicol antibiotics were intended to be used in the BHK-21 An30 cellsand the foot and mouth disease virus (FMDV) culture during the vaccine production process. Methylthiazolyldiphenyltetrazoliumbromide (MTT) and cell cultures assays were performed in the BHK-21 An30 cells treated with the antibioticsthat took place in this study to determine the concentration that inhibits cell proliferation and adverseeffects on cell morphology. Virus cultures were performed by inoculating of FMDV serotypes (A/TUR/11, O/TUR/07,Asia-1/TUR/15) to the treated cell cultures with the antibiotics. 146S and infective titres of the obtained virus suspensionswere determined. The non-toxic upper limit was determined to be 15 μg /ml for enrofloxacin and 300 μg/ml for linco-spectin in terms of cell morphology and cell numbers versus positive control (gentamycin, penicillinstreptomycin)and negative control (antibiotic-free medium) as a result of MTT and cell culture tests on BHK cells. Itwas observed that florfenicol also inhibited cell proliferation and induced cell degeneration, even at a concentrationof 5 μg/ml. The mean 146S values of FMD virus cultures containing enrofloxacin and linco-spectin were determinedto be 0.49, 0.46, 0.53, 0.47 and 0.30, 0.28 μg/ml for serotype A/TUR/11, O/TUR/07 and Asia-1/TUR/15 respectively.The mean values of the infective titres were 107,04, 107,25, 106,04, 106,59 and 107,26, 107,6 pfu/ml for serotype A/TUR/11, O/TUR/07 and Asia-1/TUR/15 respectively. In the control group used gentamycin, penicillin-streptomycinand antibiotic-free medium, the mean 146S FMD virus particles were obtained as 0.51, 0.50, 0.50; 0.52, 0.55, 0.52and 0.36, 0.33, 0.30 μg/ml for A/TUR/11, O/TUR/07 and Asia-1/TUR/15 respectively. The mean values of the FMDinfective virus titres were 107,35, 107,40, 107,11; 106,24, 106,46, 106,62, and 107,70, 107,75, 107,77 pfu/ml for A/TUR/11, O/TUR/07and Asia-1/TUR/15 respectively.As a result, the infective FMDV titer and 146S results obtained in the control group (gentamicin, penicillin-streptomycin)and FMD virus cultures using enrofloxacin (15 μg/ml) and lincospectin (300 μg/ml) were very close to eachother. According to these data, it was concluded that enrofloxacine and lincospectin can be used up to the upper limitin the BHK-21 An30 cell and FMD virus cultures. However, Florfenicol should not be used in cell and virus cultures.