Detection of extended-spectrum β-lactamase producing Escherichia coli, carbapenem and colistin resistance in chicken eggs from Istanbul

Süleymanoğlu A. A., Aydın A., Aksu H.

ACTA VETERINARIA HUNGARICA, vol.72, no.3, pp.1-20, 2024 (SCI-Expanded, Scopus)

  • Publication Type: Article / Article
  • Volume: 72 Issue: 3
  • Publication Date: 2024
  • Journal Name: ACTA VETERINARIA HUNGARICA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.1-20
  • Istanbul University-Cerrahpasa Affiliated: Yes

Abstract

ESBL-producing E. coli strains threaten public health and obligate the use of last-resort antibiotics. This study determined 15 (12.5%) E. coli isolates through 16S rRNA and gyrB genes specific to E. coli in 120 egg samples. Antibiotic resistance was detected according to the EUCAST and CLSI in E. coli isolates. 4 isolates were susceptible to all antibiotics, only 1 isolate was resistant to 2 antibiotics, and 11 E. coli isolates (73.3%) had multidrug resistance, respectively. In addition, most antibiotic resistance developed against ampicillin (80%), tetracycline (66.6%), and chloramphenicol (66.6%) in E. coli isolates. A double-disc confirmation test analyzed ESBL existence, and blaTEM, blaSHV, blaCTX-M, and blaOXA genes were searched by PCR. The blaTEM (100%) genes were found in all E. coli isolates, and blaCTX-M genes were detected in only 3 (20%) E. coli isolates. However, none of the E. coli isolates contained the genes responsible for carbapenem and colistin resistance. Our results show that multi-drug antibiotic resistance and the blaTEM gene are wide-ranging in E. coli. This is the first comprehensive study in Türkiye on ESBL-producing E. coli isolates in table eggs.