Akademik Veri Yönetim Sistemi
Letters in Drug Design and Discovery, cilt.22, sa.11, 2025 (SCI-Expanded, Scopus)
Background Hydrogels have good water retention, biocompatibility and biodegradability. As a result, their medical use has become increasingly widespread. Objective Building upon these advantages, this study aimed to prepare, characterize, and investigate the biological properties of sodium alginate hydrogels containing Astragalus campylosema extract. Methods For the characterization of hydrogels, FTIR, SEM and swelling test and for biological properties, anti-tyrosinase activity, antibacterial and cytotoxicity analyses were performed. Results The results of FTIR and SEM images were consistent with the literature. Swelling test results of all hydrogels showed that the hydrogels continued to swell until the end of the 20th hour. Cell viability in L929 fibroblast cells and dermal fibroblast (DF) cells was assessed after 12, 24, and 48 h. In L929 cells, after 12 h, the cell viability of all hydrogels was approximately 99 %. After 24 h, the cell viability of all hydrogels was approximately 92 %, and after 48 h, the cell viability percentages were approximately 90 %. Cell viability in DF cells was determined to be 97.93 % at 12 h, 94.12 % at 24 h, and 89.5 % at 48 h, and it was found that none of these values showed a cytotoxic effect. According to these results, all hydrogels were found to be biocompatible. Among bacteria, Hydrogels 1, 2, 3 and 4 had good inhibition against S. aureus and E. faecalis . Hydrogels 1, 2, 3 and 4 had lower antibacterial activity against A. baumannii (G-) and K. pneumoniae (G-). As the ratio of Astragalus extract in the hydrogel increased, tyrosinase activity in the hydrogel also increased proportionally. Conclusion Astragalus- doped sodium alginate hydrogels stood out with their biocompatible and antibacterial properties and also showed effective results in tyrosinase enzyme inhibition, indicating that they could also be successful in tyrosinase inhibition. This study showed that it would be a pioneer for in vivo studies.