Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: İstanbul Üniversitesi-Cerrahpaşa, Cerrahpaşa Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Türkiye
Tezin Onay Tarihi: 2019
Tezin Dili: Türkçe
Öğrenci: KADRİYE YAĞMUR ORUÇ
Danışman: Sibel Akyol
Özet:
Nowadays, biomaterials (Stainless
steel, Co-Cr based alloys, Titanium and titanium alloys etc.) are used in the
stabilization of musculoskeletal system to solve fractures and spinal
degeneration problems.
In our study, we analyzed the
activation of macrophages and sub groups (M1, M2a, M2b, M2c) which are the
first triggered cells in the innate immune system mechanism by implanting
Titanium and Titanium 500 into the spinal region in male Wistar albino strain, in order to control the specific inflammation
events that may occur after implantation.
21 Wistar
albino strain male rats were divided into three groups: Control (K) (n =
7), Titanium (Ti) (n = 7), Titanium 500 (Ti 500) (n = 7). Biomaterials (10 mm
diameter, 3 mm thickness) were placed between L5 and L6 region in rats.
Peripheral blood was collected on the 1st, 3rd, 5th
and 7th days. Serum CCL3, CCL4, CD163, CD206, CCL1, CD150 parameters
were analyzed with ELISA method.
When
compared to K and Ti group; increasing in CD163 on 1st day and CD206
on 5th day p<0,001***; when compared K and Ti 500 group;
decreasing in CCL1 on 1st day p<0,001***, increasing in CCL4, CD206 on 1st
day, CCL1, CCL3, CD206 on 3rd day, CD206 and CCL1 on 7th
day p<0,001***; when compared to Ti and Ti 500 group;
decreasing in CCL1, CD163 and increasing in CCL3 on 1st day p<0,001###, increasing in CCL1 on 3rd and
7th day p<0,001### in Ti
500 group was observed.
According to the results of our study, we believe that
controllable low immune responses in Ti 500 implanted patients will guide the
prevention of inflammatory reactions and development of immunological
therapeutic methods.